clinchem_example_A
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Clinical Chemistry Part A
Example question:

(Answers are at bottom of page)

  1. To 2.0 mL of a glucose solution, is added 1.0 mL of a solution containing excess ATP, NADP+, MgCl2, hexokinase and glucose-6-phosphate dehydrogenase. The change of absorbance of the final solution (in a 1 cm curvette) relative to the blank reading was 0.91 at 340 nm. The molar absorptivity of NADPH is 6.22 x 103. The concentration of glucose in the original solution is:
    1. 2.2 x 10-2 mol/L
    2. 6.22 x 10-4 mol/L
    3. 2.2 x 10-4 mol/L
    4. 6.22 x 10-3 mol/L
    5. 2.2 x 10-3 mol/L

 

Answers:1. C